Contact an Account ManagerDevelopment of a Solid-Phase-Extraction and Derivatisation Method for Polar Carboxylic Acids from Aqueous Extracts of Inorganic Multi-component Incineration Residue
Summary
Type: SPE
Application #: 302670
Catalog#: 730108G - SPE Cartridges, Chromabond, HR-P, 3ml, Glass, 200mg 30/pk
Detection: GC/MS
Conditions: Column conditioning: The SPE columns were subjected to conditioning with 3 x 1 ml methanol, followed by 3 x 1 ml water (pH 1, NaCl-saturated).
Sample application: 5 ml of the sample (about 1 mg/l per acid, < pH 1, NaCl-saturated) were loaded slowly on the cartridge. After the addition of the sample, the residual water was removed from the columns in a centrifuge at about 1000 rpm for 10 min and the columns were then dried in a stream of nitrogen for 1 min.
Elution: For elution, a Na2SO4 column was connected to the outlet of the SPE column by column adapters. Then, slow elution with 4 x 1 ml MTBE (methyl-tert.-butyl-ether) followed. The eluate was collected in pre-calibrated 5 ml V-shaped glasses.
Evaporation: The collected eluates were reduced to about 1 ml in a nitrogen flow and subjected to derivatization. This procedure allowed rapid processing without additional clean-up operations being required.
Authors: Hirschlag H Köster R
Source: Fresenius J Anal Chem (1998) 263:274-280
Details
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